Here we present Fiji Macro which detects and segments cells in a 2D monolayer by DAPI staining and quantifies the fluorescence intensity of a reporter system. It can be applied to widefield and confocal images.
Click on the image to start the tutorial video 
The original publication can be found here: https://www.sciencedirect.com/science/article/pii/S221501612200214X#ecom0004
