Cell Line
16HBE14o-; HBE (human bronchial epithelial cell); 16-HBE14o; 16HBE140; 16-HBEo; 16HBEo-; 16-HBE; 16HBE
Organism
human
Tissue/Cell type
bronchial epithelial
Genetically modified (GenTSV)
S1
Risk group (BioStoffV), sensitizing or toxic effects
1
Risk assessment
According to data sheet and based on ZKBS Az.: 6790-10-34 and Gruenert et al. 1988 (see attachment)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Merck (Charité, Daniel Lauster) | 13.04.2020 | ? | ? | MEM 10% FBS 1% P/S | 1 | Elisa Quaas | 11/01, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| Merck (Charité, Daniel Lauster) | 10.05.2019 | p2 | 1000000 | MEM (10% FBS, 1% PS, 2g/l Glutamin) | 1 | Elisa Quaas | 10/04, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free before freezing |
| Merck (Charité, Daniel Lauster) | 06.04.2020 | p6 | 1000000 | MEM (10% FBS, 1% PS, 2g/l Glutamin) | 15 | Elisa Quaas | 10/04, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free before freezing |
| Merck (Charité, Daniel Lauster) | 19.08.2020 | p36 | 1000000 | DMEM 10% FBS 1% P/S | 5 | Elisa Quaas | 10/04, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free before freezing, changed media from MEM to DMEM |
| Merck (Charité, Daniel Lauster) | 19.07.2022 | p47 | 1000000 | DMEM 10% FBS 1% P/S | 5 | Elisa Quaas | 04/07, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free before freezing |
| Merck (Charité, Daniel Lauster) | 16.01.2023 | p50 | 1000000 | DMEM 10% FBS 1% P/S | 3 | Elisa Quaas | 04/08, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) | |
| From Chuanxiong Nie | 29.01.2023 | P12 | 1000000 | DMEM 10% FBS 1% P/S | 2 | Jingyi Tang | 06/10, N2 tank (SupraFab, Altensteinstraße 23a, 114.1) |
Background
https://web.expasy.org/cellosaurus/CVCL_0112
16HBE14o- is a human bronchial epithelial cell line isolated from a 1-year old male heart-lung patient and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-). The cell line retains characteristic features of normal differentiated bronchial epithelial cells including a cobblestone morphology, cytokeratin expression, the ability to form tight junctions, and directional ion transport1. When grown with an air/liquid interface, cilia can be detected. In contrast to most other respiratory cell lines, 16HBE14o- expresses high levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein1. Expression of CFTR is correlated to cAMP-dependent Cl- conductance in a variety of cells, including 16HE14o- epithelial cells.
16HBE14o- is widely used to model barrier function of the airway epithelium in vitro and to study respiratory ion transport as well as the function of CFTR.
Cultivation and freezing protocols
check data sheet
Data sheets, further information
https://www.sigmaaldrich.com/catalog/product/mm/scc150?lang=de®ion=DE
