Location: almost every lab
Responsible Person:
Stefanie Wedepohl Tel 58721, stefanie.wedepohl@fu-berlin.de
Type of Measurement:
Precise low volume liquid dispensing
Requirements:
- Gain permission to work in the Biolabs by follwoing the How-to-Start procedure.
- Watch the pipetting video and go through the pipetting Eppendorf pipetting tutorials to learn how to prpoper and precisely pipette to obtain good results and maintain goood qualitity of the pipette.
- Get an introduction to the device by the responsible person (see above).
- For micropipettes in the Biolabs: Every workspace has their own set of pipettes, they need to stay at this place and can not be taken elsewhere
- Pipettes are for aqueous solutions only
- Disposable plastic tips need to be attached for use
- Pipettes need to be operated slowly to avoid bubbling and contamination of the inner channel
- Never lay down a pipette when a filled pipette tip is attached as this may result in liquid entering the pipette
- If liquid was drawn into the inner channel, immediately disassemble and clean it, refer to the manual for instruction.
- Pipettes need calibration and maintenance, grease and calibration tools are in the original box. There is a box with a collection of spare parts and accessories in E027 Arnimallee 22.
- Only proper dispensing technique ensures accurate volumes (e.g. always touch the vessel walls when dispensing, don't insert the tip more than a few mm into the liquid, etc. see below)
- Observe the tips during use helps avoiding errors (air bubbles, tip not attached properly, droplets outside the tip)
- report broken pipets to the responsible person, they will be collected and shipped for repair/calibration
Instruction/Manual:
(images link to the resp. manuals)
Eppendorf_Liquid-Handling_Operating-manual_Research-plus.pdf
AT-VWREHPSingleChannel(P3925).pdf
AT-VWREHPMultichannel(P4308).pdf
