Bacteria incl. Archaea
NEB® 5-alpha Competent E. coli (High Efficiency)
Strain/Type
derivative of DH5 alpha
Source/Company
NEB #C2987
Usage Regulation and Limitations
How to cite
Host tropism
Route of Transmission
Characteristics e.g. sensitizing or toxic effects, resistance to antibiotics
fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
For C2987H, C2987I, C2987P and C2987U: 1 - 3 x 109 cfu/μg pUC19 DNA
For C2987R: 1 - 5 x 108 cfu/μg pUC19 DNA
- Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
- Resistance to phage T1 (fhuA2)
- Suitable for blue/white screening by α-complementation of the β-galactosidase gene
- Reduced recombination of cloned DNA (recA1)
- K12 Strain
Approved as biological safety measure if taken as recipient organism for genetic engineering?
yes
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
Operation instructions (mandatory for RG2 and higher)
Occupational health care (according to ArbMedVV)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| NEB | NEB® 5-alpha Competent E. coli (High Efficiency) #C2987 | 27.7.2020 | nd | 8 | Stefanie Wedepohl | -80°C Omega Shelf B Rack 6 Box 1 row 5 | component of Q5 mutagenesis kit | |
