Bacteria
Escherichia coli
Strain/Type
E. coli XL1-Blue, K12 derivative
Genotype: recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacIqZΔM15 Tn10 (Tetr)]
Characteristics e.g. sensitizing or toxic effect, resistance to antibiotics
XL-1 cells are tetracycline resistant. XL1-Blue cells are endonuclease (endA) deficient, which greatly improves the quality of miniprep
DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the
EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.
Approved as biological safety measure if taken as recipient organism for genetic engineering?
yes
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/ecoli/detail.jsf?dswid=2647&dsrid=605&id=54)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | bacterial strain | freezing date | amount of bacteria per vial | host cells, stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Agilent | XL-1B | 21.1.2016 | nd | 21.1.2016 | 1 | Stefanie Wedepohl | -80°C "Omega", 115.2 Shelf B Rack 6 Box 1 H1 | glycerol stock |
| Agilent | XL-1B | var. | nd | 21.1.2016 | var. | Stefanie Wedepohl | -80°C "Omega", 115.2 Shelf B Rack 6 Box 1 row 3 | varying number of aliquots of chemically competent cells prepared from glycerol stock |
Background
nalidixic acid resistant
tetracycline resistant (carried on the F plasmid)
