Location:
Arnimallee 22, room E030
Responsible Person:
- Stefanie Wedepohl, Arnimallee 22, E001, Tel. 58721, stefanie.wedepohl@fu-berlin.de
- Daniel Kutifa, Arnimallee 22, E028, Tel. 61281, daniel.kutifa@fu-berlin.de
- Tatyana Povolotsky, Arnimallee 22, tatyana.povolotsky@fu-berlin.de
Type of measurement:
Liquid chromatography for protein purification/analysis in aqueous solutions at medium pressure (< 5 bar = 0.5 MPa) with automated fraction collector
- Application depends on column used (e.g. size exclusion, affinity, ion exchange, etc.)
- Detectors: UV (190-700 nm), conductivity
- Sample volumes: 5 µL - 5 mL (sample loop) up to several liters (pump inlet A3)
- Flow rates: 0.001 - 25 mL/min
Requirements:
- All users must gain permission to work in the Biolabs and access to the “Zellbio-users” network drive by attending the Biosafety instructions (--> Katharina Achazi)
- All users must get an introduction to the device by the responsible person
- Mark the time slot at which you want to do a measurement in the calendar, to avoid conflict with other user’s plans
- All buffers and solutions must be prepared with MilliQ, filtered (0,22µm) and degassed before use (simultaneous filtering and degassing can be done in E030)
- Read up on the properties of your column matrix (capacity, pore size, chemical resistance, max pressure, etc)
- Get familiar with the flow path
List of available columns/resins (09/2021)
- Size-exclusion columns: Superdex 200 10/300; Superdex 200 Increase 10/300; HiTrap Desalting 5 mL; HiPrep 26/60 Sephacryl S-400 HR; Superose 6 Increase 10/300
- Ion-exchange columns: several HiTrap SP Sepharose FF 1mL; several HiTrap Q Sepharose HP 5mL; HiTrap DEAE FF 1mL and 5mL; HiTrap CM FF 1mL; HiScreen Q HP 4.7mL;
- Affinity columns: HisTrap HP 1mL
- Empty columns: XK26/70, XK10/40,
- Resins: Superdex 75 PrepGrade, Q Sepharose Fast Flow 100 mL; Sephacryl S-200 HR 750 mL
→ Use the SOP to prepare the system for start and storage
Instruction/Manual:
Serial No.: 2041215
FUB Inventory No.: 72000043090
Safety Operation Instruction (German):
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