Location: Altensteinstr. 23a, room 114.3 (S2 Biolabs)
Responsible Persons & Booking: https://fub.openiris.io/timeline/d13cd388-9024-46c7-b5f6-bed0c22fa790
Detailed information and protocols how to use OpenIris you find here.
Requirements:
- Gain permission to work in the Biolabs by follwoing the How-to-Start procedure.
- Get access to the BCP-storage network drive suprafab-analytics to save measuremnt data.
- Book the device using OpenIRIS (protocls see here).
- Get an introduction to the device by the responsible person (see above).
- All buffers and solutions must be prepared with MilliQ, filtered (0,22µm) and degassed before use (simultaneous filtering and degassing can be done in 114)
- Read up on the properties of your column matrix (capacity, pore size, chemical resistance, max pressure, etc)
- Get familiar with the flow path
- → Use the SOP to prepare the system for start and storage
Type of measurement:
Liquid chromatography for protein purification/analysis in aqueous solutions at medium pressure (< 5 bar = 0.5 MPa) with automated fraction collector
- Application depends on column used (e.g. size exclusion, affinity, ion exchange, etc.)
- Detectors: UV (190-700 nm), conductivity
- Sample volumes: 5 µL - 5 mL (sample loop) up to several liters (pump inlet A3)
- Flow rates: 0.001 - 25 mL/min
List of available columns/resins (09/2021)
- Size-exclusion columns: Superdex 200 10/300; Superdex 200 Increase 10/300; HiTrap Desalting 5 mL; HiPrep 26/60 Sephacryl S-400 HR; Superose 6 Increase 10/300
- Ion-exchange columns: several HiTrap SP Sepharose FF 1mL; several HiTrap Q Sepharose HP 5mL; HiTrap DEAE FF 1mL and 5mL; HiTrap CM FF 1mL; HiScreen Q HP 4.7mL;
- Affinity columns: HisTrap HP 1mL
- Empty columns: XK26/70, XK10/40,
- Resins: Superdex 75 PrepGrade, Q Sepharose Fast Flow 100 mL; Sephacryl S-200 HR 750 mL
Instruction/Manual:
Serial No.: 2041215
FUB Inventory No.: 72000043090
Safety Operation Instruction (German):
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