Cell Line

T1-α-D842V+G680R, T1-a-G680R-K2

Organism

Homo sapiens (Human) 

Tissue/Cell type

Gastrointestinal stromal tumor (NCIt: C3868)

Gastrointestinal stromal tumor (ORDO: Orphanet_44890)

Derived from metastatic site: Pleural effusion.

Genetically modified (GenTSV)

Yes, see S1 Arbeit 2: S1 Projects Formblatt Z (92/14)

Risk group (BioStoffV), sensitizing or toxic effects

1

Risk assessment

see Formblatt Z (facility 92/14 project 2): S1 Projects Formblatt Z (92/14) and cells are tested negative for HIV, HBV and HCV (see attached email) and therefore according to ZKBS "Stellungnahme der ZKBS zur Einstufung gentechnischer Arbeiten mit primären Zellen aus Vertebraten" risk level 1

Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)

sourcefreezing datepassage no.cell count per vialcell culture medium incl. supplementsno. of aliquotsbelongs to
(full name)
rack/box in N2 tank or -80°C freezer and  location (room, address)

comments


Dr. Thomas Mühlenberg (Tel.: 0201 723 3150), S1-Anlage der Inneren Klinik und Poliklinik, Az 64-0-1.55/04; Anl.-Nr. 95306.07.2023px+5~ 1Mio
IMDM (10% FBS, 1% P/S, 1% Amphoterecin)10Elisa Quaas08/02, N2 tank (SupraFab, Altensteinstraße 23a, 114.1)

Background

Cultivation and freezing protocols

Data sheets, further information

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