Eukaryotic Microorganism
Leishmania tarentolae, Tarentola mauritanica
Strain/Type
LEXSY host T7-TR, Parasitic protozoan
Host tropism (if pathogenic)
Not pathogenic: The unicellular kinetoplast protozoan Leishmania tarentolae, isolated from the Moorish gecko Tarentola mauritanica, not pathogenic to mammalians (Biosafety level 1)
Route of Transmission (if pathogenic)
Not pathogenic
Characteristics e.g. sensitizing or toxic effects, resistance to antibiotics, parasite, infectious
Light sensitive, have to be cultured in the dark at 26 °C with hemin supplementation under aerobic conditions, L. tarentolae will not survive 37 °C
Approved as biological safety measure if taken as recipient organism for genetic engineering?
Risk group (BioStoffV)
RG1
Risk assessment
Risk accessment based on TRBA (Technical Rule for Biological Agents) 464 "TRBA 464 Einstufung von Parasiten in Risikogruppen": https://www.baua.de/DE/Angebote/Regelwerk/TRBA/TRBA-464.html
Occupational health care (according to ArbMedVV)
In the case of activities with the biological agent no health care is required. An indication on restrictions for immunosuppressed workers should be made.
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | eukaryotic microorganism | freezing date | amount of eukaryotic microorganisms per vial | stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Jena Bioscience | LEXSY host T7-TR | 01.08.2023 | 4x106 cells/mL | Anja Knorrscheidt Daniel Lauster | R115 Omega: C-3-1 | |||
Background
Cultivation and freezing protocols
https://www.jenabioscience.com/lexsy-expression/lexsy-background/download-methods
Data sheets, further information
https://zag.bvl.bund.de/organismen/detail.jsf?dswid=3069&dsrid=692&id=1086
