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Location: Altensteinstr. 2323a, 114.4 (S2 Biolabs)
Responsible Person:
Persons & Booking: https://fub.openiris.io/timeline/3581469e-cab4-47df-b3ef-f69aba1e61c8
Detailed information and protocols how to use OpenIris you find here.
Requirements:
- Gain permission to work in the Biolabs by follwoing the How-to-Start procedure.
- Get access to the BCP-storage network drive suprafab-analytics to save measuremnt data.
- Book the device using OpenIRIS (protocls see here).
- Get an introduction to the device by the responsible person (see above).
Type of MeasurementType of measurement:
Flow cytometry (FCM) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer.
Uses for flow cytometry include:
- Cell counting
- (Cell sorting)
- Determining cell characteristics and function
- Detecting microorganisms
- Biomarker detection
- Protein engineering detection
- Diagnosis of health disorders such as blood cancers
- Note: Cell sorting is not possible with this device
A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties.
This flow cytometer is equipped with 2 lasers:
1) 488nm 2) 640nm
and 4 detectors:
FL1: 533/30 nm (e.g. FITC/GFP)
FL2: 585/40 nm (e.g. PE/PI)
FL3: > 670 nm (e.g. PerCP, PerCP-Cy™5.5, PE-Cy7)
FL4: 675/25nm (e.g. APC)
Requirements:
- Before use, Marie Weinhart needs to be contacted to get the permission to use the device
- All users must gain permission to work in the Biolabs and access to the “Zellbio-users” network drive by attending the Biosafety instructions (--> Katharina Achazi)
- All users must get an introduction to the device by the responsible person
- Booking via OpenIRIS
3 lasers and several detectors.
Instruction/Manual:
Software: Analysis PC & Software
1-Training manual and User Guides
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name | 100032905_Attune_NxT_Auto_Sampler_UG_RevA.pdf |
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name | 100037944_Attune_NxT_EFS_QR_RevA.pdf |
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name | 100038577_Attune_NxT_EFS_UG_RevA.pdf |
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height | 250 |
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name | Attune Flow Cytometry Maintenance and Troubleshooting Guide.pdf |
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height | 250 |
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name | AttuneNxTBasicTrainingGuide_rev2.5.pdf |
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height | 250 |
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name | 100024233_Attune_NxT_QR_RevA.pdf |
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height | 250 |
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Instruction/Manual: View file |
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name | BD100024235_AccuriAttuneNxT_C6FlowHW_CytoUG_Instrument_ManualRevC.pdf |
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height | 250 |
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name | 100024236_Attune_NxT_SW_UG_RevJ.pdf |
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height | 250 |
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name | 100024428_AttuneNxTSitePrepGuide_RevB.pdf |
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height | 250 |
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2-Application Notes
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name | CO017307-MurineRegulatoryTcells-AppNote-AttuneNxT.pdf |
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height | 250 |
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name | CO017309 0815-App-Note-Attune-NxT-Human-10-Color.pdf |
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height | 250 |
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name | CO018965-LiveDead Algal-AttuneNxt-AppNote.pdf |
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height | 250 |
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name | COL02200 Field Guidelines Carryover-AttuneNxT.pdf |
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height | 250 |
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name | COL02204-FluorescentProtein-AppNote-AttuneNxT.pdf |
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height | 250 |
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name | COL13402-Concentration-AttuneNxT-FieldGuidelines.pdf |
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height | 250 |
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name | COL31386-FlowCytometryDoseResponseApoptosis-AppNote.pdf |
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height | 250 |
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name | COL31388-ModFitLT-AppNote-AttneNxT.pdf |
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height | 250 |
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name | COL31523 0417-whole-blood-attunenxt-flowcytometer-appnote.pdf |
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height | 250 |
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name | COL31525-Tcell10colorPanel-AppNote-AttuneNxT.pdf |
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height | 250 |
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name | COL31961-FlowCytometryHpscCardiomyocytes-AppNote.pdf |
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height | 250 |
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name | COL32178-HighThroughputAutomation-AppNote-AttuneNxT.pdf |
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height | 250 |
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name | COL32264-FCSExpress-AppNote-AttuneNxT.pdf |
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height | 250 |
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name | COL32542-AnalysisNuclearDNAContentPlants-AttuneNxT-AppNote.pdf |
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height | 250 |
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name | COL32649-FlowJo-AppNote-AttuneNxT.pdf |
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height | 250 |
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name | COL32973-TCellBackbonePanel-AppNote-AttuneNxT.pdf |
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height | 250 |
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name | CO016291 0715-human-13-color-attune-nxt-appnote.pdf |
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height | 250 |
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name | CO017293 0815-detection-leukocytes-attune-nxt-appnote.pdf |
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height | 250 |
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name | CO017301 0915-detection-phagocylic-cells-phrodo-bioparticle.pdf |
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height | 250 |
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name | CO017303 0815-App-Note-Attune-NxT-No-Compensation-Panels.pdf |
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height | 250 |
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name | CO017305 0915-App-Note-Attune-NxT-Mouse-10-color-with-foxp3.pdf |
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height | 250 |
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3-Additional Documents
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name | Attune NxT - Daily Maintenance.pdf |
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height | 250 |
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name | Attune NxT Autosampler validated plates.pdf |
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height | 250 |
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name | CO014745-RemoteDiagnostics-FAQ.pdf |
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height | 250 |
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name | CO019173-InstrumentManagement-Brochure.pdf |
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height | 250 |
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name | COL06884-MaintenanceTroubleshootingQRC-AttuneNxT.pdf |
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height | 250 |
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name | COL18696-ConnectSecurity-WhitePaper.pdf |
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height | 250 |
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name | COL26183-2022-Fluorophore-Selection-Guide-Poster.pdf |
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height | 250 |
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name | DataManagement-AttuneNxTSoftwareBD_Accuri_C6_Software_User_Guide.pdf |
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height | 250 |
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name | Attune Cytometric Software v5.1.1 Release Notes.pdf |
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height | 250 |
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4-Additional Protocols
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name | CO23134-0611 Bacterial analysis.pdf |
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height | 250 |
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name | manual live dead baclight viability kit.pdf |
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height | 250 |
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name | Attune Daily Maintenance.pdf |
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height | 250 |
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Serial No.:AC6531180424
FUB Inventory No.: 72000036890
Safety Operation Instruction (German):
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