Cell Line
Jurkat-NFKB-GFP-Artv1 / Jurkat-NFKB-GFP-Betv1
Organism
human
Tissue/Cell type
T-cell
Genetically modified (GenTSV)
Yes (S1)
Risk group (BioStoffV)
1
Risk assessment
According to Formblatt Z, Gentechnische Abeit Nr. 3, Anlage 92/14 and according to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=27&dswid=7455&dsrid=98) and Miguel Alvaro-Benito (FU Berlin, AG Freund), see here
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Miguel Alvaro-Benito (FU Berlin, AG Freund) | 24.6.2020, 1.7.2020 | Px+3, Px+5 | 5E6 | RPMI incl. glutamin 10% FBS 1& P/S | 2,10 | Stefanie Wedepohl | Rack 5 Box 4, N2 Tank (SupraFab, Altensteinstraße 23a, 114.1) | suspension cells |
Background
Jurkat T-cell GFP reporter cell line with TCR specific to Artv1 or Betv1 peptide
Cultivation and freezing protocols
cultivate between between 0.3 and 2 Mio cells/ml, avoid clumps, freeze 3-5Mio cells/ml
