Here we present Fiji Macro which detects and segments cells in a 2D monolayer by DAPI staining and quantifies the fluorescence intensity of a reporter system. It can be applied to widefield and confocal images.
Click on the image to start the tutorial video 
The macro can be downloaded here: Download
The original publication can be found here: https://doi.org/10.1016/j.mex.2022.101834
