Flow Cytometer Thermo Fisher (Attune NxT)

Location:

Altensteinstr. 23a, 114.4

Responsible Person:

see OpenIRIS (protocls see here)

Type of measurement:

Flow cytometry (FCM) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer.

Uses for flow cytometry include:

Cell counting
(Cell sorting)
Determining cell characteristics and function
Detecting microorganisms
Biomarker detection
Protein engineering detection
Diagnosis of health disorders such as blood cancers

A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties.

This flow cytometer is equipped with 2 lasers:

1) 488nm 2) 640nm

and 4 detectors:

FL1: 533/30 nm (e.g. FITC/GFP)
FL2: 585/40 nm (e.g. PE/PI)
FL3: > 670 nm (e.g. PerCP, PerCP-Cy™5.5, PE-Cy7)
FL4: 675/25nm (e.g. APC)

Requirements:

All users must gain permission to work in the Biolabs and access to the “Zellbio-users” network drive by attending the Biosafety instructions (--> Katharina Achazi)
All users must get an introduction to the device by the responsible person (see OpenIRIS)
Booking via OpenIRIS (protocls see here)

Instruction/Manual:

1-Training manual and User Guides