In this section you will get an overview of the difference between raw images (e.g., 8- or 16-bit images in czi- or lif-format) and processed images (e.g., RGB-images in png- or tif-format). In general, the images you take at the microscope should be exported as raw images (czi, lif, ...) and not as processed images (png, jpeg, ...). The raw images contain important information like original pixel intensites and meta data (like pixel size, objective magnification, used lasers, and gain) and can be used for quantitative analysis and to be processed (e.g., color adjustment and insertion of a scale bar) and exported as figures (e.g. as RGB-images in png-format).
Additional information can be found here:
