Virus
Herpes simplex virus type 1
Herpes simplex-Virus Typ 1
HHV-1
Strain/Type
HSV-1-GFP
(F-strain)
Genus: Simplexvirus
Source/Company
Freie Unibeversität Berlin, Department of Veterinary Medicine, Institute of Virology, Prof. Benedikt Kaufer (PhD) and Dr. med. vet. Jakob Trimpert
Usage Regulation and Limitations
For publications, acknowledge the Freie Unibeversität Berlin, Department of Veterinary Medicine, Institute of Virology, Prof. Benedikt Kaufer (PhD) and Dr. med. vet. Jakob Trimpert and cite the publications listed in the next paragraph.
How to cite
For publications, refer to this two publications:
Tanaka M, Kagawa H, Yamanashi Y, Sata T, Kawaguchi Y. Construction of an excisable bacterial artificial chromosome containing a full-length infectious clone of herpes simplex virus type 1: viruses reconstituted from the clone exhibit wild-type properties in vitro and in vivo. J Virol. 2003 Jan;77(2):1382-91. doi: 10.1128/jvi.77.2.1382-1391.2003. PMID: 12502854; PMCID: PMC140785.
Brunialti M, Höfler T, Nascimento M, Trimpert J. Suicidal Phenotype of Proofreading-Deficient Herpes Simplex Virus 1 Polymerase Mutants. J Virol. 2023 Jan 31;97(1):e0135922. doi: 10.1128/jvi.01359-22. Epub 2023 Jan 4. PMID: 36598203; PMCID: PMC9888220.
Host tropism
Humans.
Zoonosis (transmission between animals and humans): Yes
Primates as well as rabbits and rodents can be infected, but do not represent a natural reservoir for human herpes viruses.
Route of Transmission
Transmission takes place percutaneously (through the skin).
Transmission takes place orally (by ingestion).
Characteristics e.g. sensitizing or toxic effects, replication deficiant, not infectious, attenuated, inactivated (upload relevant documents)
Human-pathogenic (causes diseases in humans).
Approved as biological safety measure if taken as recipient organism for genetic engineering?
Genetically modified (GenTSV)
yes
construct: Tanaka M, Kagawa H, Yamanashi Y, Sata T, Kawaguchi Y. Construction of an excisable bacterial artificial chromosome containing a full-length infectious clone of herpes simplex virus type 1: viruses reconstituted from the clone exhibit wild-type properties in vitro and in vivo. J Virol. 2003 Jan;77(2):1382-91. doi: 10.1128/jvi.77.2.1382-1391.2003. PMID: 12502854; PMCID: PMC140785.
GFP insert: Brunialti M, Höfler T, Nascimento M, Trimpert J. Suicidal Phenotype of Proofreading-Deficient Herpes Simplex Virus 1 Polymerase Mutants. J Virol. 2023 Jan 31;97(1):e0135922. doi: 10.1128/jvi.01359-22. Epub 2023 Jan 4. PMID: 36598203; PMCID: PMC9888220.
Risk group (BioStoffV)
2
Risk assessment
Risk accessment based on TRBA (Technical Rule for Biological Agents) 462 "Classification of viruses into risk groups": https://www.baua.de/DE/Angebote/Rechtstexte-und-Technische-Regeln/Regelwerk/TRBA/TRBA-462.html
Operation instructions (mandatory for RG2 and higher)
https://biostoffe.dguv.de/data?name=830562&lang=en
Occupational health care (according to ArbMedVV)
Optional health care:
In the case of tasks specifically involving contact and tasks involving incidental contact with biological agents classed as Risk Group 2 under the Biological Agents Ordinance (Biostoffverordnung, BioStoffV) or which involve a comparable risk, the employer must offer an optional health care. This does not apply when on account of the risk assessment and on account of the protective measures taken it can be assumed that there is no risk of infection.
An optional health care must also be offered if as a result of the exposure to biological agents
- a serious infectious illness is to be expected and post-exposure prophylatic measures are possible, or
- an infection has resulted.
Vaccination:
None available.
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | virus strain | freezing date | virus amount per vial | stock was produced on | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°C freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| Freie Unibeversität Berlin, Department of Veterinary Medicine, Institute of Virology, Prof. Benedikt Kaufer (PhD) and Dr. med. vet. Jakob Trimpert | HSV-1-GFP (F-strain) | 2023.01.23 | 2.4 x 10^7 PFU/mL, 1mL | Vero | 9 | Ranen Etouki/Chuanxiong Nie | C21, -80oC freezer, Π (SupraFab, Altensteinstraße 23a, 114.1) | discarded on 21.04.2023 |
| Freie Unibeversität Berlin, Department of Veterinary Medicine, Institute of Virology, Prof. Benedikt Kaufer (PhD) and Dr. med. vet. Jakob Trimpert | HSV-1-GFP (F-strain) | 2023.01.23 | 7.6 x 10^6 PFU/mL, 1mL | Vero | 9 | Yanping Long/Chuanxiong Nie | C21, -80oC freezer, Π (SupraFab, Altensteinstraße 23a, 114.1) | discarded on 21.04.2023 |
| Freie Unibeversität Berlin, Department of Veterinary Medicine, Institute of Virology, Prof. Benedikt Kaufer (PhD) and Dr. med. vet. Jakob Trimpert | HSV-1-GFP (F-strain) | 2023.04.27 | 10^7 PFU/mL, 1mL | Vero | 9 | Chuanxiong Nie | C29, -80oC freezer, Π (SupraFab, Altensteinstraße 23a, 114.1) | |
| Freie Unibeversität Berlin, Department of Veterinary Medicine, Institute of Virology, Prof. Benedikt Kaufer (PhD) and Dr. med. vet. Jakob Trimpert | HSV-1-GFP (F-strain) | 2023.05.05 | 10^8 PFU/mL, 1mL | Vero | 9 | Chuanxiong Nie | C29, -80oC freezer, Π (SupraFab, Altensteinstraße 23a, 114.1) | |
| Freie Unibeversität Berlin, Department of Veterinary Medicine, Institute of Virology, Prof. Benedikt Kaufer (PhD) and Dr. med. vet. Jakob Trimpert | HSV-1-GFP (F-strain) | 2023.06.29 | 2.1x10^6 PFU/mL, 1mL | Vero | 8 | Chuanxiong Nie | C29, -80oC freezer, Π (SupraFab, Altensteinstraße 23a, 114.1) | |
| Freie Unibeversität Berlin, Department of Veterinary Medicine, Institute of Virology, Prof. Benedikt Kaufer (PhD) and Dr. med. vet. Jakob Trimpert | HSV-1-GFP (F-strain)
| 2023.02.11 | 4 x 10^8 PFU/mL 1mL | Vero E6 | 10 | Chuanxiong Nie | C29, -80oC freezer, Π (SupraFab, Altensteinstraße 23a, 114.1) | |
| Freie Unibeversität Berlin, Department of Veterinary Medicine, Institute of Virology, Prof. Benedikt Kaufer (PhD) and Dr. med. vet. Jakob Trimpert | HSV-1-GFP (F-strain) | 2023.08.18 | 2x10^7 PFU/mL, 1mL | Vero E6 | 9 | Yanping Long | C21, -80oC freezer, Π (SupraFab, Altensteinstraße 23a, 114.1) | |
| Freie Unibeversität Berlin, Department of Veterinary Medicine, Institute of Virology, Prof. Benedikt Kaufer (PhD) and Dr. med. vet. Jakob Trimpert | HSV-1-GFP (F-strain) | 2024.02.07 | 2.45 x 10^8 PFU/mL 5 x 10^8 PFU/mL | Vero E6 | 8 x 1ml, 1 x 0.2ml 14 x 50µl clean 1ml x uncleaned | Latifa Abadel | C-2-6, -80oC freezer, Π (SupraFab, Altensteinstraße 23a, 114.1) |
Background
Cultivation and freezing protocols
Data sheets, inactivation cetificate, further information
HSV-1-GFP (F-strain)
Tanaka M, Kagawa H, Yamanashi Y, Sata T, Kawaguchi Y. Construction of an excisable bacterial artificial chromosome containing a full-length infectious clone of herpes simplex virus type 1: viruses reconstituted from the clone exhibit wild-type properties in vitro and in vivo. J Virol. 2003 Jan;77(2):1382-91. doi: 10.1128/jvi.77.2.1382-1391.2003. PMID: 12502854; PMCID: PMC140785.
GFP insertion
Brunialti M, Höfler T, Nascimento M, Trimpert J. Suicidal Phenotype of Proofreading-Deficient Herpes Simplex Virus 1 Polymerase Mutants. J Virol. 2023 Jan 31;97(1):e0135922. doi: 10.1128/jvi.01359-22. Epub 2023 Jan 4. PMID: 36598203; PMCID: PMC9888220.
