Seitenhistorie

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• Gain permission to work in the Biolabs by follwoing the How-to-Start procedure.
• Get access to the BCP-storage network drive suprafab-analytics to save measuremnt data.
• Book the device using OpenIRIS (protocls see here).
• Get an introduction to the device by the responsible person (see above).
• All buffers and solutions must be prepared with MilliQ, filtered (0,22µm) and degassed before use (simultaneous filtering and degassing can be done in E030)
• Read up on the properties of your column matrix (capacity, pore size, chemical resistance, max pressure, etc) 
  • Size exclusion chromatography column selection guide.
  • Affinity chromatography media selection guide
  • Ion exchange chromatography media selection guide
• Get familiar with the flow path
• → Use the SOP to prepare the system for start and storage

Type of measurement:

Liquid chromatography for protein purification/analysis in aqueous solutions  at medium pressure (< 5 bar = 0.5 MPa) with automated fraction collector

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• Size-exclusion columns: Superdex 200 10/300; Superdex 200 Increase 10/300; HiTrap Desalting 5 mL; HiPrep 26/60 Sephacryl S-400 HR; Superose 6 Increase 10/300 
• Ion-exchange columns: several HiTrap SP Sepharose FF 1mL; several HiTrap Q Sepharose HP 5mL; HiTrap DEAE FF 1mL and 5mL; HiTrap CM FF 1mL; HiScreen Q HP 4.7mL;  
• Affinity columns: HisTrap HP 1mL
• Empty columns: XK26/70, XK10/40, 
• Resins: Superdex 75 PrepGrade, Q Sepharose Fast Flow 100 mL; Sephacryl S-200 HR 750 mL

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Instruction/Manual:

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