Location: Altensteinstr. 23a, 114.4
Responsible Person:
(S2 Biolabs)
Responsible Persons & Booking: https://fub.openiris.io/timeline/3581469e-cab4-47df-b3ef-f69aba1e61c8
Detailed information and protocols how to use OpenIris you find here.
Requirements:
- Gain permission to work in the Biolabs by follwoing the How-to-Start procedure.
- Get access to the BCP-storage network drive suprafab-analytics to save measuremnt data.
- Book the device using see OpenIRIS (protocls see here).
- Get an introduction to the device by the responsible person (see above).
Type of measurementMeasurement:
Flow cytometry (FCM) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer.
Uses for flow cytometry include:
- Cell counting
- (Cell sorting)
- Determining cell characteristics and function
- Detecting microorganisms
- Biomarker detection
- Protein engineering detection
- Diagnosis of health disorders such as blood cancers
- Note: Cell sorting is not possible with this device
A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties.
This flow cytometer is equipped with 2 lasers:
1) 488nm 2) 640nm
and 4 detectors:
FL1: 533/30 nm (e.g. FITC/GFP)
FL2: 585/40 nm (e.g. PE/PI)
FL3: > 670 nm (e.g. PerCP, PerCP-Cy™5.5, PE-Cy7)
FL4: 675/25nm (e.g. APC)
Requirements:
- All users must gain permission to work in the Biolabs and access to the “Zellbio-users” network drive by attending the Biosafety instructions (--> Katharina Achazi)
- All users must get an introduction to the device by the responsible person (see OpenIRIS)
- Booking via OpenIRIS (protocls see here)
3 lasers and several detectors.
Instruction/Manual:
Software: Analysis PC & SoftwareInstruction/Manual:
1-Training manual and User Guides
...
