Location: Altensteinstr. 23a, 115.1 (S2 Biolabs)
Responsible Persons & Booking: https://fub.openiris.io/timeline/ff370255-1cde-45d4-a40b-5039848e358c
Detailed information and protocols how to use OpenIris you find here.
Requirements:
- Get permission from Marie Weinhart (marie.weinhart@fu-berlin.de) to use the device.
- Gain permission to work in the Biolabs by follwoing the How-to-Start procedure.
- Get access to the BCP-storage network drive suprafab-analytics to save measuremnt data.
- Book the device using OpenIRIS (protocls see here).
- Get an introduction to the device by the responsible person (see above).
Type of Measurement:
Flow cytometry (FCM) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer.
Uses for flow cytometry include:
- Cell counting
- (Cell sorting)
- Determining cell characteristics and function
- Detecting microorganisms
- Biomarker detection
- Protein engineering detection
- Diagnosis of health disorders such as blood cancers
A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties.
This flow cytometer is equipped with 2 lasers:
1) 488nm 2) 640nm
and 4 detectors:
FL1: 533/30 nm (e.g. FITC/GFP)
FL2: 585/40 nm (e.g. PE/PI)
FL3: > 670 nm (e.g. PerCP, PerCP-Cy™5.5, PE-Cy7)
FL4: 675/25nm (e.g. APC)
Instruction/Manual:
Serial No.: AC6531180424
FUB Inventory No.: 72000036890
Safety Operation Instruction:
see BAs Devices
