Location:
Altensteinstr. 23a, 114.4
Responsible persons & booking: https://fub.openiris.io/timeline/3581469e-cab4-47df-b3ef-f69aba1e61c8
Detailed information and protocols how to use OpenIris you find here.
Type of measurement:
Flow cytometry (FCM) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer.
Uses for flow cytometry include:
- Cell counting
- Determining cell characteristics and function
- Detecting microorganisms
- Biomarker detection
- Protein engineering detection
- Diagnosis of health disorders such as blood cancers
- Note: Cell sorting is not possible with this device
A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties.
This flow cytometer is equipped with 3 lasers and several detectors.
Requirements:
- Gain permission to work in the Biolabs by follwoing the How-to-Start procedure.
- Get access to the BCP-storage network drive suprafab-analytics to save measuremnt data.
- Book the device using OpenIRIS (protocls see here).
- Get an introduction to the device by the responsible person (see above).
Instruction/Manual:
Software: Analysis PC & Software
1-Training manual and User Guides
2-Application Notes
3-Additional Documents
4-Additional Protocols
Serial No.:
FUB Inventory No.:
Safety Operation Instruction (German):