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Leica SP5

Location: Königin-Luise-Str. 12-16, 14195 Berlin, AG Wiermer

SN: 

FUB: 


Responsible persons & booking: https://fub.openiris.io/timeline/dcc6fb09-bd5e-4fa5-b711-2b13637cb765

Detailed information and protocols how to use OpenIris you find here.


Requirements:

  • Gain permission to work in the OM facilty.
    • Get access to the BCP-storage network drive suprafab-analytics to save measuremnt data; in addtion, provide (a) User name, (b) ZEDAT account (c) Research lab& (d) Device name to the device responsible person (see above) to get access to the device specific folder

    e.g. device specific training & safety briefing:

    1. Contact the repsonsible person shown in OpenIris (https://fub.openiris.io/timeline/dcc6fb09-bd5e-4fa5-b711-2b13637cb765) to get an an introduction & training to the device.
      Follow the addtional info for full accesss on the Access to the Optical Microscopy (OM) Facility page.
    2. Book the device
    using
    1. using OpenIRIS (protocls see here
    ).Get an introduction to the device by the responsible person (see above
    1. ).


    Confocal Laser Scanning Microscope Leica SP5

    Confocal laser scanning microscopy enables optical sectioning of multilayer fluorescent specimens with high contrast by applying a pinhole in the optics. It is therefore possible to image a thin optical slice out of a thick specimen (up to 100 µm) that represents under optimal conditions a slice of approximately 500 nm. Moreover, conventional contrast methods such as the differential interference contrast (DIC) can be used and in reflection mode particles or surfaces can be analyzed.


    Detailed specifications CLSM Leica SP8 (1)

    For more information contact device Admins (AG Wiermer).