Location: Altensteinstr. 23a (SupraFAB), lab 027.1 (S2 area)
Details:
The sample preparation lab is dedicated to the direct preparation of living samples for optical micvrscopy as cells, plants and other organsisms. It is equipped with
- a basic light microscope & a basic stereo microscope for preparation of e.g. larvae (coming soon)
- micropipettes
Micropipettes (Eppendorf/VWR/various) - a pipette boy
Pipette controller PIPETBOY acu2 (INTEGRA) - a fridge/freezer combi (in the ale 026)
- two climate chambers for plants & a CO2 incuabtor for eucaryotic cells
Incubator with CO2 for eucaryotic cells C170/CB-S170/321 (Binder/Thermo Scientific) - a water bath and
- a sterile work bench (booking via OpenIris: https://fub.openiris.io/timeline/e9c339df-2608-4439-b6b7-91d279525b79 (Detailed information and protocols how to use OpenIris you find here.)
- a basic light microscope
- pipettes
- a fridge/freezer combi
- two climate chambers for plants
- a CO2 incuabtor for eucaryotic cells
- a water bath and
- Bench Biosafety Cabinet Class II Safe2020 (Thermo Scientific)
- a small benchtop centrifuge
Centrifuge Heraeus Megafuge 8/8R (Thermo Fisher Scientific)a small benchtop centrifuge
Requirements e.g. device specific training & safety briefing:
- Register as new SupraFAB user by sending personal info (name, working group, status/activity, office and laboratory room number) to safety@suprafab.fu-berlin.de, and participate in person in the next SupraFAB safety briefing (usually first Monday of the month, 10:00 - 12:00, you should receive infos by email).
In case you DO NOT receive info about the next SupraFAB safety briefing, watch the 3 safety briefing videos (available here: https://wikis.fu-berlin.de/x/pQhVT) on your own. - Fill the transponder form with work safety questionaire(2. page), let it sign by your research group head and the respective responsible persons in supraFAB and finally hand in to Katharina Tebel (office 103) or Achim Wiedekind (office 102).
- Contact the responsible person shown in OpenIris (https://fub.openiris.io/timeline/e9c339df-2608-4439-b6b7-91d279525b79) to get an an introduction & training to the lab.
- Follow the addtional info for full accesss on the Access to the Optical Microscopy (OM) Facility page.
- Book the device using OpenIRIS (protocls protocols see here).
Note: Scientific guests as well as non-scientific staff such as service personal from companies also need to get introduced by one of the PIs for genetic engeeneering (see Biolab Team & Lab Phone Numbers). The introducution and documentation forms and slides can be found on the safety intro page (https://wikis.fu-berlin.de/x/pQhVT). The documentation forms and permission forms need to be stored in the repsective folder in office 111.