Location:
Altensteinstr. 23a (SupraFAB), lab 026.2 (OM S2 area)
SN:3967000224
FUB:
Responsible persons & booking: https://fub.openiris.io/timeline/ba8e4fb9-4cf9-43c7-8a4e-ad5b96d41a57
Detailed information and protocols how to use OpenIris you find here.
Requirements e.g. device specific training & safety briefing:
- Register as new user via the SupraFAB Homepage participate in person in the SupraFAB safety briefing (see SupraFAB Homepage); & become familiar with the safety guidelins, instructions and rules & biolab agreement.
Fill the transponder form with work safety questionaire(2. page), let it sign by your research group head and the respective responsible persons in supraFAB and finally hand in to Katharina Tebel (office 103) or Achim Wiedekind (office 102).
- Make an appointment with your Keyuser*, to get practical training (2-3 session) & an intro to the OM, the BioSupraMol, data handling (network) as well as the SupraFAB House guidelines.
In case you are the first person of your group who wants to use the device or in case of fuurtehher questions, contact the responsible person shown in OpenIris (https://fub.openiris.io/timeline/ba8e4fb9-4cf9-43c7-8a4e-ad5b96d41a57). - Follow the addtional info for full accesss on the Access to the Optical Microscopy (OM) Facility page.
- Book the device using OpenIRIS (protocls see here).
Note: Scientific guests as well as non-scientific staff such as service personal from companies also need to get introduced by one of the PIs for genetic engeeneering (see Biolab Team & Lab Phone Numbers). The introducution and documentation forms and slides can be found on the safety intro page (https://wikis.fu-berlin.de/x/pQhVT). The documentation forms and permission forms need to be stored in the repsective folder in office 111.
Zeiss Axio Zoom.V16 Stereo Microscope
The Axio Zoom.V16 fluorescence stereomicroscope has a 16x zoom and a high numerical aperture of NA 0.25, making it suitable for viewing large samples and whole model organisms in fluorescence contrast and reflected light.
Suitable for
Large samples and whole model organisms
Fluorescently stained and unstained samples
Microscope Details
Objectives: Objektiv Plan Z 1,0x/0,25 FWD 60mm mit Auflösung von 100 ... 740 LP/mm oder kleinstem Detail 5,0 ... 0,7 μm; Abgleichlänge 164mm, Frontgewinde M62x0,75
Illumination:
- Fluorescence: Beleuchtungseinrichtung HXP 200C
- Brightfield: LED-Doppelspot
Fluorescent filter cubes (for preview by eye pieces)::
- Filterset 38 Endow GFP shift free (E) EX BP 470/40, BS FT 495, EM BP 525/50
- Filterset 45 HQ TexasRed, d=25 shift free (E) EX BP 560/40, BS FT 585, EM BP 630/75
Detection: Mikroskopie-Kamera Axiocam 305 mono
Software: ZEN 3.6 mit Apeer onsite advanced - Basic 3D viewing - Connect basic - Manual EDF - Measurement - Panorama - Spectral unmixing - ZEN Data Storage Client - Acquisition Base (time series and multichannel)
Manufacturer: Zeiss
Zeiss Axio Zoom.V16 Stereo Microscope
Das Fluoreszenz-Stereomikroskop Axio Zoom.V16 hat einen 16-fach-Zoom und eine hohe numerischen Apertur von NA 0,25. Es eignet sich daher für die Betrachtung großer Proben und ganzer Modellorganismen im Fluoreszenzkontrast und Auflicht.
Geeignet für
- Große Proben und ganze Modellorganismen
- Fluoreszenzgefärbte und ungefärbte Proben
