Bacteria
Escherichia coli (RG1)
Strain/Type
Escherichia coli B- and K12-derived strains:
- E.coli 4TUB93
- E.coli 5TUB83
- E.coli 6TUB128-OC
- E.coli 6TUB165-MB3
- E.coli 6TUB165-MB4
- E.coli 7TUB165-MB
- E.coli 7TUB165-OC
- E. coli AR3110
- E. coli BL21(DE3)
- E. coli BL21(DE3) Ril
- E. coli BL21(DE3) RIPL
- E. coli BL21(DE3) TrpRS
- E.coli C2566
- E. coli DH5a
- E. coli DH10B
- E. coli Ind-TUB165
- E. coli K12 (BAM237)
- E. coli NEB® 5-alpha Competent
- E. coli NEB Stable
- E. coli ORN178
- E. coli ORN208
- E. coli TUB00
- E. coli W-TUB165
- E. coli XL1-Blue
Host tropism
Humans, various animal species.
Route of Transmission
Transmission takes place percutaneously (through the skin) and orally (by ingestion).
Zoonosis (transmission between animals and humans): Yes
Characteristics e.g. sensitizing or toxic effects, resistance to antibiotics
Check in the strain list above and in the ZKBS datbase for each strain: https://www.zkbs-online.de/ZKBS/EN/Databases/Recipient%20strains%20for%20biological%20safety%20measures_Strains/Recipient%20strains%20for%20biological%20safety%20measures_node.htmlApproved as biological safety measure if taken as recipient organism for genetic engineering?
Check in the ZKBS datbase for each strain: https://www.zkbs-online.de/ZKBS/EN/Databases/Recipient%20strains%20for%20biological%20safety%20measures_Strains/Recipient%20strains%20for%20biological%20safety%20measures_node.html
Genetically modified (GenTSV)
If yes, documentation in Formblatt Z needed: Formblatt Z (S1 & S2) & Project Applications (S2)
Risk group (BioStoffV)
1
Risk assessment
Risk accessment based on TRBA (Technical Rule for Biological Agents) 466 "Classification of prokaryotes (bacteria and archaea) into risk groups": https://www.baua.de/DE/Angebote/Rechtstexte-und-Technische-Regeln/Regelwerk/TRBA/TRBA-466.html
and the "Statement of the ZKBS on the suitability of Escherichia coli K12-derived strains as part of biological safety measures according to § 8 para. 1 GenTSV" and the "Stellungnahme der ZKBS zur Einstufung von Escherichia coli C als Spender- und Empfängerorganismus bei gentechnischen Arbeiten zu Forschungszwecken".
Occupational health care (according to ArbMedVV)
Optional health care:
In the case of tasks specifically involving contact and tasks involving incidental contact with biological agents classed as Risk Group 2 under the Biological Agents Ordinance (Biostoffverordnung, BioStoffV) or which involve a comparable risk, the employer must offer an optional health care. This does not apply when on account of the risk assessment and on account of the protective measures taken it can be assumed that there is no risk of infection.
An optional health care must also be offered if as a result of the exposure to biological agents
- a serious infectious illness is to be expected and post-exposure prophylatic measures are possible, or
- an infection has resulted.
Vaccination:
No approved vaccine is available.
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
see specific strains.
